Variegata strain help

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madhouse

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Hi folks I wonder if anyone can help I have grown Variegata tobacco for a few years and have had to take it of my seed list because of unpredicted results.
<O:p</O:p
The plants I have grown this year don’t seem to have the variegation they should I don’t understand plant genetics that well but have tried different ways of getting the variegation back.
<O:p</O:p
Things like bagging one plant keeping 2 heavily variegated plants together, keeping 2 plants with variegated parents but showing no variegation together and so on, but I am only averaging about 5% variegation in seedling.
<O:p</O:p
I would love to keep this strain up and running if for no reason other than this is a glorious looking plant when full grown and it smokes well to, any help would be great.
<O:p</O:p
Bill :confused: <O:p</O:p
 

Hasse SWE

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If no one else will help you so I can help you Bill. But I would like to see someone a little closer to help you.
 

skychaser

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Variegation:

http://en.wikipedia.org/wiki/Variegation

Variegation is the appearance of differently coloured zones in the leaves, and sometimes the stems, of plants. This may be due to a number of causes. Some variegation is attractive and ornamental and gardeners tend to preserve these. The term is also sometimes used to refer to colour zonation in flowers, minerals, and the skin, fur, feathers or scales of animals. ....

Chimeral:

Plants bearing such variegation are chimeras, with more than one type of genetic makeup in their tissues. A lack of chlorophyll producing tissue in some tissues causes variegation with white or yellow coloured zones on the leaf, contrasting with the usual green tissue. It is due to some of the plant’s meristematic tissue losing the ability to produce chloroplasts, so that the tissue it produces is no longer green.

In a common type of such variegation, the part of the meristem that produces epidermal tissue loses the ability to produce chloroplasts. The margins of leaves may be composed only of cells derived from this merismatic tissue. Hence, this marginal tissue is white or yellow, rather than green. There are several other types of such variegation, depending on the tissues that have been affected, and their relationship to each other. Variegation may be consistent and symmetric in appearance throughout the whole plant, or it may be quite random in location. In some plants, entire branches or stems including the leaves may be variegated. The variegation in some forms is unstable. The extent and nature of the variegation can vary, and sometimes the plant will return to the green form. In others it is stable and does not change under normal conditions.

Because the variegation is due to the presence of two kinds of plant tissue, propagating the plant must be by a vegetative method of propagation that preserves both types of tissue in relation to each other. Typically, stem cuttings, bud and stem grafting, and other propagation methods that results in growth from leaf axil buds will preserve variegation. Cuttings with complete variegation may be difficult if not impossible to propagate. Root cuttings will not usually preserve variegation, since the new stem tissue is derived from a particular tissue type within the root.

As these plants have some of their tissue unable to carry out photosynthesis, the plant will be weaker than the plain green plant. They should generally be expected to die out in nature and their only real source of survival is through cultivation." .....

Also see this: Chimera (plant)

http://en.wikipedia.org/wiki/Chimera_(plant)

Chimeras (or chimaeras) in botany are usually single organisms composed of two genetically different types of tissue. They occur in plants, on the same general basis as with animal chimeras. However, unlike animal chimeras, both types of tissues may have originated from the same zygote, and the difference is often due to mutation during ordinary cell division. .....

Propagation

Because chimeras have more than one type of genetic material, while they may produce viable offspring from seed, these will not be true to type. All propagation that preserves the variation has to be by cuttings or division. Some types of cuttings, such as root cuttings, will produce entirely new growing points, usually from the inner one of the two types of tissue, so that these cannot be used either."
 

Hasse SWE

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Bill not sure I understand you right now. But yes you have already filled my farm for a year.Plan to make a growing blog. in the blog I thought I'd follow the five variants. They should show the plant with flowers, with a bag over the flowers and topped.Later I also dry tobacco in different ways. Everything to show People that you can do both easier and more difficult for themselves to grow tobacco. When it comes to location, my wife's dad promised me a extra piece of land that I haven't yet imagined till then at where it can get to grow. But like I said, prefer that someone else will help you.

//Hasse
 

Dean

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Variegation is a gene. So the seed will carry that gene but not always show it, that is why you only get 5%. Just sow more seed and grow on the best.

Some will not carry the gene and be normal.

you cannot make more come out or make every plant show it.

cheers
 

madhouse

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Many thanks Sky you are a mine of knowledge and thanks to everyone else.

Ok I have done quite a bit of reading on this subject and it seems there are several of ways to induce variegation into a tobacco plant, firstly by means of a harmless virus, not a good choice.

Secondly buy means of heavily ion beam on the seed, oh yes I have one of them just lying in the garage.

And thirdly by passing the gene on from the parent plant, from what I have read using Turkish stains won’t work as they inhibit the gene from being passed on ?,
Also any cross pollination from variegated and self colour plants is a no-no as the green pigment dominates, but if im reading it right self pollination of a variegated parent plant will produce 95% or there about’s green self coloured seedlings, ok that’s not good but what happens next could be.

The gene seems to be passed on in the egg cell of the plant and does not show in the next generation, but if these self coloured plants are self pollinated again the resulting progeny should show variegation at a rate of about 60-40% variegation.

If anyone has more info on this I would love to hear it and if any one would like to do a grow out on this I have seed that has gone through the first stage and will produce self coloured green plants which will need to be bagged and raised again to see if the variegation comes out.

Bill
 

Dean

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Spent half of my life breeding orchids and trying to get my head around their genetics. My understanding would be from a self pollination of a variegated plant roughly
25% var
50% split for var May or may not show
25% normal

by selfing and keeping track of that first 25% you will slowly increase the number of variegated plants but it will take many generations to breed true.

%ages are rough but would be pretty close to what you would expect with many other species of plant in a first generation cross.

cheers
 
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